Western blot was performed using Anti-CABIN1 Polyclonal Antibody (Product # PA5-60313) and a 246 kDa band corresponding to Calcineurin-binding protein cabin-1 was observed across all the cell lines tested along with uncharacterized bands (*). Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), IMR-90 (Lane 2), HUVEC (Lane 3), HCT 116 (Lane 4), SH-SY5Y (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).